Person: ATAK, ÇİMEN
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ATAK
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ÇİMEN
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Publication Metadata only Effect of magnetic field on peroxidase activities of soybean tissue culture(DIAGNOSIS PRESS LTD, 67 DONDUKOV BLVD, 1504 SOFIA, BULGARIA, 2007-05) Olgun, Atok; Alikamanoğlu, Sema; Rzakoulieva, A.; ATAK, ÇİMEN; ÇELİK, ÖZGE; TR6653; TR141172In this study, the aim was to determine the effect of magnetic field on peroxidase activities of soybean tissue culture. Shoot tips were put into petri dishes and exposed to a magnetic field for a period of 2.2 and 19.8 s at a magnetic flux of 2.9-4.6 mT. The shoot and root formation rate, fresh weights, chlorophyll quantities, total RNA concentrations and peroxidase activities of regenerated shoots from control and treated shoot tips were determined. While the rate of shoot formation was 28.57% in the control group, this rate was increased to 94.33% and 78.18%, respectively, in the explants that were exposed to a magnetic field for a period of 2.2 and 19.8 s. While the percentage of root formation in controls was 4.76%, this rate increased to 47.17% and 54.54%, respectively, in those that were exposed to a magnetic field at the same periods. When the fresh weights were determined, we found that the fresh weights of plantlets regenerated from treated explants were increased relative to controls. Chlorophyll a, chlorophyll b and total chlorophyll contents increased 21%, 13% and 18%, respectively, relative to control groups at 2.2 s. Peroxidase activity significantly increased in all magnetic field treatments (p<0.05). The total RNA concentration of seedlings regenerated from treatment explants significantly increased relative to controls (p<0.05). The regeneration and plant growth of shoot tips exposed to a magnetic field with a 2.2 s period were positively affected by the MF and increased with respect to controls and the length of time exposed.Publication Metadata only Induced of plastid mutations in soybean plant (Glycine max L. Merrill) with gamma radiation and determination with RAPD(2004-11-22) Alikamanoğlu, Sema; Açık, Leyla; Canpolat, Yasemin; ATAK, ÇİMEN; 6653; 176641; 1781The aim of our study was to induce with radiation of atrazine resistant and tolerated mutants in Coles, Amsoy-71 and 1937 soybean varieties. Atrazine that is photosynthetic inhibitor is the most important herbicide of S-triazin group, and shows toxic effect on soybean plant. For the improvement of the atrazine resistant plants with mutation breeding, the seeds belonging to the three varieties were irradiated with 200 Gy of gamma radiation dose. The irradiated seeds were sown in the field and at the end of harvesting season, every pod at node situated on the main stem was picked up separately and M2 generations were obtained. At the plants, which were obtained from M2 generation, chlorophyll mutants were determined and atrazine selection was made. The percentage of chlorophyll mutants for Amsoy-71, Coles and 1937 soybean varieties were found as 1.07, 1.48 and 1.32, respectively. At the end of atrazine selection, the percentages of atrazine resistant plants for Amsoy-71, Coles and 1937 soybean varieties were 0.80, 0.60 and 0.53, respectively. The percentages of atrazine tolerated plants were 1.07, 1.18 and 1.05, respectively as well. In our research; the differences among the mutants replying to atrazine in various concentrations were examined by using RAPD procedure as the molecular marker techniques in comparison with polymorphism. In the study done by using 14 primers; according to the amplification results, the differences between atrazine resistant plants were shown.Publication Metadata only Radiosensitivity in callus tissues of soybean(glycine max.(L.)merril)(1994) Alikamanoğlu, Sema; ATAK, ÇİMEN; 6653; 176641Radiosensitivity of soybean as expressed by callus growth was examined after exposure of primary leaf explants with gamma rays.The leaf explants were irradiated 24 hours after incubation on the medium with gamma radiation doses of 0,5,7.5,10,25,50,75 and 100 Gy from Co-60 source. At the 30-day culture of callus the percentage of callus formation from irradiated explants was not different from the percentage of control callus formation. The decreasing average callus fresh weights were in connection with the increasing doses of the radiation and this relationship was significant (P<0.05).The differences between the doses of 5 Gy with 50 Gy and 100 Gy were significant (P<0.05) and the Gr-50 dose was found as 37 Gy for J-357 soybean variety.But the comparison of close doses of radiation was not significant from the point of decreasing average callus fresh weightPublication Metadata only Soya fasülyesi Glycine Max L Merrill kallus ve meristem kültürlerine gama radyasyonunun etkileri(1996) Alikamanoğlu, Sema; Yalçın, S.; ATAK, ÇİMEN; 6653; 176641Publication Metadata only Effect of magnetic field on Paulownia seeds(2000) Yurttaş, B.; Alikamanoğlu, Sema; Topçul, F.; Rzakoulieva, Aytekin; Danilov, V.; ATAK, ÇİMEN; 6653; 176641Publication Embargo Stimulation of regeneration by magnetic field in soybean (Glycine max L. Merrill) tissue cultures.(Haliç University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Fındıkzade 34280, İstanbul-Turkey, 2003) Emiroğlu, Özge; Alikamanoğlu, Sema; Rzakoulieva, Aytekin; ATAK, ÇİMEN; 6653; 176641The determination of the effects of magnetic field on tissue cultures will be helpfull for the magnetic field treatments. In this study which was begun with this purpose, the seeds belonging to J 357 soybean variety were used. The soybean seeds were germinated in sterile conditions and cultures were initiated from the shoot tips. The explants in petri dishes were exposed to 2.9-4.6 mT magnetic fields for 2.2, 6.6, and 19.8 seconds periods. Shoot and root formation rate, fresh weights and chlorophyll quantities of regenerated shoots from control and treated explants were determined. While the shoot formation was 61.91% in control group, this rate increased in all magnetic field experiments and this rate raised up to 86.96% and 74.36% respectively in the explants which were exposed to magnetic field at 2.2 and 6.6 seconds periods. Again, while the percentage of root formation in control was 14.29%, this rate raised up to 26.08% and 35.90% respectively in these which were exposed to magnetic field at the same periods. When the fresh weights were determined, the fresh weights of seedlings regenerated from treatment explants from 6.6 seconds significantly increased in accordance with control (P<0.05). Chlorophyll a, chlorophyll b and total chlorophyll quantities of shoot leaf were increased at the treatment magnetic field for 2.2 secondsPublication Metadata only Manyetik Alanın Soya (Glycine max L.Merrill) Doku Kültürleri Üzerine Etkisi(2003-08-25) Emiroğlu, Özge; Alikamanoğlu, Sema; Rzakoulieva, Aytekin; ATAK, ÇİMEN; 6653; 113987; 176641Publication Metadata only Manyetik Alanın In Vitro Soya Büyümesinde Peroksidaz Aktivitesi ve Total RNA Miktarı Üzerine Etkisi(2004) Olgun, Atok; Emiroğlu, Özge; Alikamanoğlu, Sema; ATAK, ÇİMEN; 6653; 113987; 176641Publication Metadata only Soya fasulyesi Glycine Max L Merrill Meristem kültürlerinde gama radyasyonunun bitki rejenerasyonuna etkisi(1994) Alikamanoğlu, Sema; ATAK, ÇİMEN; 6653; 176641Publication Metadata only Effect of magnetic field and gamma radiation on Paulowinia tomentosa tissue culture(DIAGNOSIS PRESS LTD, 67 DONDUKOV BLVD, 1504 SOFIA, BULGARIA, 2007-02) Alikamanoğlu, Sema; Yaycılı, Orkun; Rzakoulieva, A.; ATAK, ÇİMEN; TR6653; TR176641; TR151085In this study Me in vitro tissue culture of the Paulownia tomentosa plant was established and a magnetic field which has 2.9-4.8mT flux density was applied to the culture at a velocity of 1m/s for 19.8 seconds. Additionally, gamma radiation of 10 and 25 Gy was applied as combined to the magnetic field. It was observed that magnetic field increased the capability of regeneration of Paulownia tomentosa cultures and supported the regeneration in a short time in comparison with the control group and applied radiation dose and decreased regeneration capability were re-supported when combined with magnetic field. It was found that magnetic field has a positive effect on plant fresh weight, leaf number and chlorophyll amount on the 28(th) day of established cultures Q P tomentosa node explants and the negative effects on these parameters by radiation, when magnetic field and gamma radiation applied together these parameters changed and aroused depending on the radiation doses.